Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus species

Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species.

BACKGROUND/AIM To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. MATERIALS AND METHODS DNA was isolated...

In vitro metacestodicidal activity of genistein and other isoflavones against Echinococcus multilocularis and Echinococcus granulosus

corresponding authors: Andrew Hemphill, Arunasalam Naguleswaran Institute of Parasitology, Vetsuisse Faculty, University of Berne Länggass-Strasse 122 CH-3012 Berne, Switzerland Phone +41 31 6312384 Fax +41 31 6312477 Email [email protected] [email protected] AC CE PT ED Copyright © 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Antimic...

In vitro metacestodicidal activities of genistein and other isoflavones against Echinococcus multilocularis and Echinococcus granulosus.

Echinococcus multilocularis and Echinococcus granulosus metacestode infections in humans cause alveolar echinococcosis and cystic echinococcosis, respectively, in which metacestode development in visceral organs often results in particular organ failure. Further, cystic hydatidosis in farm animals causes severe economic losses. Although benzimidazole derivatives such as mebendazole and albendaz...

Study of Echinococcus granulosus and Echinococcus multilocularis infections in canidiae in Ardabile province of Iran

Study of Echinococcus Granulosus and Echinococcus Multilocularis Infections in Canidiae in Ardabile Province of Iran

In a cross-sectional study (1992-1994) on wild and domestic carnivores infected with E. granulosus and E. multilocularis in plain and mountaineous areas of Ardabile province, 130 intestinal specimens of dogs, red fox(Vulpes vulpes), wolves (Canis bupus), jackals (Canis aureus), wild and domestic cats (Felis chaou) were collected and examined for Echinococcus species. The percentage of E. granul...